Journal: Journal of Clinical Medicine

Article Title: Heavy Water (D 2 O) Containing Preservation Solution Reduces Hepatic Cold Preservation and Reperfusion Injury in an Isolated Perfused Rat Liver (IPRL) Model

doi: 10.3390/jcm8111818

Figure Lengend Snippet: Dsol ameliorates histopathology. H&E staining (20× and 40× magnifications) showed almost normal appearance in the CT group. Vacuolization was shown in the UW group, whereas it was suppressed in the Dsol group. TdT-mediated dUTP nick end labeling (TUNEL)-positive cells were observed in the UW group, whereas they were hardly shown in the CT and Dsol groups.

Article Snippet: Moreover, we stained frozen sections with a fluorescent TdT-mediated dUTP nick end labeling (TUNEL) staining kit (Promega, WI, USA) in accordance with the manufacturer’s instructions, described briefly in the previous report [ ].

Techniques: Histopathology, Staining, End Labeling, TUNEL Assay

Journal: BioMed Research International

Article Title: Excess Hydrocortisone Hampers Placental Nutrient Uptake Disrupting Cellular Metabolism

doi: 10.1155/2018/5106174

Figure Lengend Snippet: Assessment of hydrocortisone-induced cellular death by apoptosis in healthy-term placental explants. (a) Representative microphotographs of TUNEL assay to determine apoptosis in each experimental condition; cells undergoing apoptosis present fluorescent green nuclei. (b) Quantification of the % of TUNEL positive cells per condition. (c) Active caspases 3 and 7 as measured by luminometry, using the Caspase Glo Assay. (d) Cleaved caspase 3 as determined by western blot and normalized by actin levels. Values are presented as means ± SEM for 5 independent experiments. ∗ p≤0.05; ∗∗ p≤0.01; ∗∗∗ p≤0.001 relative to control.

Article Snippet: Apoptosis was assessed with a commercially available fluorescent terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling (TUNEL) kit (Dead-End TM Fluorometric TUNEL System Kit; Promega, Madison, WI, USA).

Techniques: TUNEL Assay, Caspase-Glo Assay, Western Blot, Control